Members of the Krogan Lab Mass Spec team are experts in a variety of proteomics techniques.

We perform a wide variety of proteomics techniques including: unbiased global proteome and PTM characterization with label-free quantitation, large-scale quantitative affinity purification mass spectrometry (AP-MS) to define protein networks, targeted quantitation via selected reaction monitoring (SRM), structural studies using cross-linking mass spectrometry (XL-MS), as well as proximity labeling mass spectrometry (APEX-MS) to study short-lived interactions (such as signaling networks) and resolve the cellular location of interactions using spatial references.
A primary interest in the lab is combining several of these complementary MS-based approaches in a systems biology fashion to develop a comprehensive picture of the biological system of interest. We perform all our MS-based experiments as part of the Thermo Fisher Scientific Proteomics Facility for Disease Target Discovery.