We are applying our expertise also to identify key regulators of cardiac differentiation. This work will help us understand how healthy heart tissue develops, how this process can go awry and lead to disease, and can hopefully in the future guide therapies and cures for heart disease. While previous studies have gained important insight into the cell state transition by focusing on transcriptional regulators and epigenetics, our work focuses on the relatively uncharacterized protein and post-translational levels. By combining data on transcription, translation, and post-translational modifications, we will produce a map of cardiac differentiation at unprecedented detail. This work is done in collaboration with investigators at the Gladstone Institute of Cardiovascular Disease (GICD).